What should not be done when collecting a lactic acid sample?

When collecting a lactic acid sample, it is essential to draw the sample without stasis. Stasis refers to the state of balance or inactivity, in this context, it typically means applying a tourniquet or other forms of compression that can affect the sample. When collecting a lactic acid sample, if stasis is applied for too long, it may result in localized increase of lactic acid in the blood, leading to inaccurate test results.

Collecting the sample without stasis ensures that the blood is circulating properly, thus providing a true representation of the lactic acid levels in the systemic circulation. This is crucial because lactic acid is often measured to assess tissue perfusion and anaerobic metabolism, and any deviation caused by stasis could complicate both diagnosis and treatment.

In contrast, storing the sample on ice, using a gray top tube, and cleaning the site with alcohol are all standard procedures that help ensure the integrity and reliability of the lactic acid measurement. Proper handling and preparation of the sample are critical for accurate laboratory results.