Which of the following can be a reason for tube failure?

A tube can experience failure due to a manufacturing defect or expiration, which means that the tube might not perform as expected if it was made with substandard materials or if it exceeded its shelf life. Manufacturing defects can lead to issues such as leaks, incorrect vacuum levels, or compromised sterility that can ultimately affect the quality of the specimen collected. When a tube is expired, the additives within it that are necessary for proper specimen preservation may become ineffective, leading to potential sample degradation or inaccurate results.

While improper handling during collection, insufficient blood volume, and improper storage temperature are all valid concerns in the context of sample collection and handling, they are considered external factors that can affect the specimen rather than the inherent integrity of the tube itself. In contrast, a defect or expiration directly relates to the quality control measures taken during the production of the tube, making it a primary reason for tube failure.